MeltingPlot is a tool for strains clustering/typing and epidemiological investigation using High Resolution Melting (HRM) temperatures. Please note: The version of MeltingPlot implemented in this page is MeltingPlot v1.0. MeltingPlot v2.5 is available on GitHub We are currently working to implement the newer version on this page. Download the template spreadsheet, fill it and start your analysis! Consider performing a test run of MeltingPlot with the example spreadsheet. You can also download the MeltingPlot Manual. The stand-alone version of the tool is available on GitHub. (Required) Your email address (we will send you the results!) Enter a valid e-mail address (Required) Upload here the input spreadsheet that you prepared from the template spreadsheet DO NOT ALTER any column name or sheet name from the template Accepted file extention are: .xls No space character accepted in the file's name Download the form and fill it WITHOUT ALTERING ANY COLUMN NAME OR SHEET NAME Did you add temperatures to the Reference_isolates sheet in the input .xls spreadsheet?Did you add data to the "Reference_isolates" sheet? No Yes Did you add data to the Isolate_metadata sheet in the input .xls spreadsheet?Did you add data to the "Isolates_metadata" sheet? No Yes You can use the Reference isolates' temperatures of Klebsiella pneuominae on wzi gene (primers and protocol in Perini et al. 2020 and Pasala et al. 2020) on the following Real-Time PCR instrument models: Illumina ECO RealTime Biorad CFX Applied Biosystem QuantStudio 6 Flex By clicking "Submit" MeltingPlot will start to run and a summary of your request will be sent to your email address After clicking "Submit" you can close this window the output folder will be sent to you or you can wait here for the output links Adavance Settings: Number of PCR replicates number of repilicats (each sample, each primer set) PNG figures resolutionPDF files are always provided. PNG files are optional and their resolution in Pixels Per Inch (PPI) can be set here. None (PDF only - fastest) 80 PPI (good resolution - fast) 120 PPI (better resolution) 180 PPI (best resolution - slow) Minimum of days to have thicker links in patients graphIn the patients' graph rhicker links will be shown when two strains where collected from the two patient in less than the days indicated here Betweenness value treshold for undetermined isolatesThis is the normalized betwennes value above which an isolate will be considered as "undetermined" in the analysis. HIGHER BETWENNESS = UNCERTAIN CLUSTERING By clicking "Submit" MeltingPlot will start to run and a summary of your request will be sent to your email address After clicking "Submit" you can close this window the output folder will be sent to you or you can wait here for the output links If you are using MeltingPlot for an article or scientfic publication please cite: Perini, M., et al. MeltingPlot, a user-friendly online tool for epidemiological investigation using High Resolution Melting data. BMC Bioinformatics 22, 76 (2021). https://doi.org/10.1186/s12859-021-04020-y The example input spreadsheet used for the publication is here To report bugs or for assistance please contact matteo.perini@unimi.it
