EasyPrimer assists primer design in difficult contexts, e.g. on an alignment of hundreds of sequences and/or on hypervariable genes.
The tool uses as input the fasta sequence of an alignment and identifies the best regions for primer design: two low variable regions flanking a highly variable one
EasyPrimer is also on GitHub https://github.com/MatteoPS/EasyPrimer
To report bugs please contact firstname.lastname@example.org
If you are using EasyPrimer for an article or scientfic publication please cite:
Perini, M., Piazza, A., Panelli, S. et al. EasyPrimer: user-friendly tool for pan-PCR/HRM primers design. Development of an HRM protocol on wzi gene for fast Klebsiella pneumoniae typing. Sci Rep10, 1307 (2020). https://doi.org/10.1038/s41598-020-57742-z